Chitra Thakur

Chitra Thakur

Title

Assistant Professor (Research)

Department

Pharmaceutical Sciences

Phone

313-577-1047

Email

chitra.thakur@wayne.edu

Chitra Thakur

Degrees and Certifications

  • 2012-Doctor of Philosophy (Ph.D.), Lung Cancer Metastasis
    University of Wurzburg / Max Planck Institute of Biochemistry, Germany 
  • 2006-Master of Science, Microbiology
    Department of Microbiology, Barkatullah University Bhopal, India
  • 2004-Bachelor of Science
    Govt. Motilal Science College, Barkatullah University Bhopal, India 

Workshops and symposiums

  • EMBO Workshop on “Human evolution and diseases’’ December, 6 - 9, 2006. 
  • INDO–US Workshop on “Mitochondrial Research and Medicine” January 22 - 24, 2007. 
  • 10th ADNAT Convention, a Two Day Symposium on “RNAi and microRNA in Development and Health”, February 23 - 24, 2006. 
  • International Symposium on” Chromosomes to Genome”, July 3 - 5, 2007. 
  • Graduate Day of the 2nd Congress of the German Society for Stem Cell Research, October 3, 2007, Am Hubland, Universität Würzburg. 
  • 3rd International Symposium organized by the students of Graduate School Of Life Science, University of Wurzburg, 8th October 2007. 
  • EMBO Workshop “Can epigenetics influence reprogramming and metastatic progression?’’ October 6-9, 2008 in Banz Monastery, Bad Staffelstein, Germany.
  • MRS-AACR Joint Conference on Metastasis and the Tumor   Microenvironment, September 12th -15th, 2010 at the Sheraton Center City, Philadelphia, PA, USA.
  • 2016 National Postdoctoral 14th Annual Meeting, March 4-6 206,Van Andel Research Institute in Grand Rapids, Michigan, USA
  • AACR Precision Medicine Series: Opportunities and Challenges of Exploiting Synthetic Lethality in Cancer. January 4 - 7, 2017. Hard Rock Hotel San Diego, California

Trainings

  • Compact-course on laboratory animals, animal experiments and alternatives (FELASA)
    Conducted by: Charite-Universitatsmedizin Berlin and ZEBE, Germany
    Certified by: GV- SOLAS (Gesellschaft fur Versuchtierkunde-Society of Laboratory Animal Science)
  • Light Microscopy for Biologists 
    Conducted by: Max Planck Institute of Biochemistry, Core Facility
  • Proteomics and Mass Spectrometry 
    Conducted by: Max Planck Institute of Biochemistry, Core Facility
  • Ethics, Science and Bioethics, Genetic testing
  • Applied Statistics for Life Scientists

Positions and Employment

  • 04/2019-present: Research Assistant Professor, Wayne State University
  • 05/2017-03/2019: Research Associate, Wayne State University
  • 09/2012-04/2017: Post-Doctoral Fellow, Wayne State University
  • 10/2007-04/2012: Graduate School of Life Sciences Fellow at the University of Wurzburg / Max Planck Institute of Biochemistry, Germany
  • 05/2006-04/2007: Project Research Fellow, Center for Cellular and Molecular Biology, Hyderabad, India

Editorial Board Member of Scientific Journals

  • Cancer and Clinical Oncology
  • American Journal of Biomedical and Life Sciences
  • Science Research Association, SCIREA Journal of Biology
  • Technology in Cancer Research & Treatment (Associate Editor)

Scientific Journal Review

  • Medicinal Research Reviews
  • Scientific Reports
  • WIREs Nanomedicine and Nano biotechnology
  • Colloids and Surfaces B: Biointerfaces
  • Molecules
  • Toxicology and Applied Pharmacology
  • Cancer and Clinical Oncology
  • Breast Cancer: Targets and Therapy
  • Current Biomarker Findings
  • Lung Cancer: Targets and Therapy
  • Oncotargets and Therapy
  • Journal of Toxicology and Environmental Health

Teaching Experience

  • Wayne State University Department of Pharmaceutical Sciences:
    • PSC 7010 - Fall, 2015
    • PSC 7010 - Fall, 2016
    • PSC 7040 - Winter, 2017
    • PSC 4115 - Fall, 2018
  • Wayne State University:
    • GS 0900 - Responsible Conduct of Research (breakout sessions) - 2016, 2017 and 2018

Awards and Honors

  • 2017: Selected for the 2nd Annual NIH-Funded Boot Camp on "Big Data Training for Translational Omics Research" Purdue University, West Lafayette, IN
  • 2017: Travel award for the 2nd Annual NIH-Funded Boot Camp on "Big Data Training for Translational Omics Research" Purdue University, West Lafayette, IN
  • 2016: Outstanding poster award, Annual Research symposium at Wayne State University
  • 2015: Outstanding poster award, Annual Research symposium at Wayne State University
  • 2007: DFG German Excellence Initiative award for doctoral studies 
  • 2006: University topper in Microbiology, Master of Science of the year
  • 2004: College topper Bachelor of Science of the year

Professional Memberships

  • 2019-Ex-officio advisory member of the Wayne State Post-Doctoral Association (WSU-PDA)
  • 2018-Chair of the Wayne State Post-Doctoral Association (WSU-PDA)
  • 2015-Member of the National Post-Doctoral Association
  • 2015-2017 Vice President of the Wayne State Post-Doctoral Association (WSU-PDA)
  • 2015-2016 Chair of the Professional Development Committee of the WSU-PDA
  • 2011- Associate member, American Association for Cancer Research
  • 2006- Member, Association of the Microbiologist of India

Areas of Expertise

  • Isolation, identification and characterization of the microorganisms
  • Transgenic mouse technology
  • Paraffin embedding, sectioning and staining of the tissues, histology
  • Modelling tumor and metastasis in-vivo
  • Invitro assays for molecular oncology studies
  • Biochemistry, cell and molecular biology tools in studying toxicology and environmental carcinogens induced tumors
  • Epigenetic mechanisms in human lung and breast cancer

Recent Publications

  1. Thakur C. Lineage tracing of metastasis in a mouse model for Non-small cell lung cancer (NSCLC), Untersuchung metastatischer Prozesse durchgenetische Zellmarkierung in einem Mausmodelldes nichtkleinzelligen Lungenkarzinoms (NSCLC) 2012. https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/6991
  2. Thakur C, Wolfarth M, Sun J, Zhang Y, Lu Y, Battelli L, et al. Oncoprotein mdig contributes to silica-induced pulmonary fibrosis by altering balance between Th17 and Treg T cells. Oncotarget. 2015;6(6):3722-36. PMID: 25669985
  3. Thakur C, Chen F. Current understanding of mdig/MINA in human cancers. Genes Cancer. 2015;6(7-8):288-302. PMID: 26413213
  4. Thakur C, Lu Y, Sun J, Yu M, Chen B, Chen F. Increased expression of mdig predicts poorer survival of the breast cancer patients. Gene. 2014;535(2):218-24. PMID: 24309373
  5. Thakur C*, Rapp UR, Rudel T. Cysts mark the early stage of metastatic tumor development in non-small cell lung cancer. Oncotarget. 2018; 9:6518-6535. (*Corresponding author) 
  6. Thakur C*. (2018). Chapter 2 - Angiogenesis in Brain Tumors. In: Kesharwani P and Gupta U, eds. Nanotechnology-Based Targeted Drug Delivery Systems for Brain Tumors: Academic Press), pp. 27-47. (*Corresponding author)
  7. Thakur, C. and F. Chen, Connections between metabolism and epigenetics in cancers. Semin Cancer Biol, 2019. 57: p. 52-58. 
  8. Q Zhang, C Thakur, J Shi, J Sun, Y Fu, P Stemmer, F Chen New discoveries of mdig in the epigenetic regulation of cancers. Semin Cancer Biol, 2019. 57: p. 27-35. (Equal contribution)
  9. L Li, Z Bi, P Wadgaonkar, Y Lu, Q Zhang, Y Fu, C Thakur, L Wang, F Chen, Metabolic and epigenetic reprogramming in the arsenic-induced cancer stem cells. Semin Cancer Biol, 2019. 57: p. 10-18.
  10. Thakur, C*.(2019). Chapter 2-An Overview, Current Challenges of Drug Resistance, and Targeting Metastasis Associated With Lung Cancer. In: Kesharwani P ed. 21-38. (*Corresponding author)
  11. Ceteci F, Xu J, Ceteci S, Zanucco E, Thakur C, Rapp UR. Conditional expression of oncogenic C-RAF in mouse pulmonary epithelial cells reveals differential tumorigenesis and induction of autophagy leading to tumor regression. Neoplasia. 2011;13(11):1005-18. PMID: 22131876
  12. Ceteci F, Ceteci S, Zanucco E, Thakur C, Becker M, El-Nikhely N, et al. E-cadherin controls bronchiolar progenitor cells and onset of preneoplastic lesions in mice. Neoplasia. 2012;14(12):1164-77. PMID: 23308049
  13. Oak PS, Kopp F, Thakur C, Ellwart JW, Rapp UR, Ullrich A, et al. Combinatorial treatment of mammospheres with trastuzumab and salinomycin efficiently targets HER2-positive cancer cells and cancer stem cells. Int J Cancer. 2012;131(12):2808-19. PMID: 22511343
  14. Kopp F, Hermawan A, Oak PS, Ulaganathan VK, Herrmann A, Elnikhely N, Thakur C, Xiao Z, Knyazev P, Ataseven B, Savai R, Wagner E, Roidl A. Sequential Salinomycin Treatment Results in Resistance Formation through Clonal Selection of Epithelial-Like Tumor Cells. Transl Oncol. 2014;7(6):702-11. PMID: 25500079
  15. Wu K, Chang Q, Lu Y, Qiu P, Chen B, Thakur C, et al. Gefitinib resistance resulted from STAT3-mediated Akt activation in lung cancer cells. Oncotarget. 2013;4(12):2430-8. PMID: 24280348
  16. Chang Q, Chen B, Thakur C, Lu Y, Chen F. Arsenic-induced sub-lethal stress reprograms human bronchial epithelial cells to CD61 cancer stem cells. Oncotarget. 2014;5(5):1290-303. PMID: 24675390
  17. Li L, Qiu P, Chen B, Lu Y, Wu K, Thakur C, et al. Reactive oxygen species contribute to arsenic-induced EZH2 phosphorylation in human bronchial epithelial cells and lung cancer cells. Toxicol Appl Pharmacol. 2014;276(3):165-70. PMID: 24582688
  18. Sun J, Yu M, Lu Y, Thakur C, Chen B, Qiu P, et al. Carcinogenic metalloid arsenic induces expression of mdig oncogene through JNK and STAT3 activation. Cancer Lett. 2014;346(2):257-63. PMID: 24434654
  19. Yu M, Sun J, Thakur C, Chen B, Lu Y, Zhao H, et al. Paradoxical roles of mineral dust induced gene on cell proliferation and migration/invasion. PLoS One. 2014;9(2). PMID: 24505346
  20. Chen B, Yu M, Chang Q, Lu Y, Thakur C, Ma D, et al. Mdig de-represses H19 large intergenic non-coding RNA (lincRNA) by down-regulating H3K9me3 and heterochromatin. Oncotarget. 2013;4(9):1427-37. PMID: 23965803
  21. Wu K, Li L, Thakur C, Lu Y, Zhang X, Yi Z, et al. Proteomic Characterization of the World Trade Center dust-activated mdig and c-myc signaling circuit linked to multiple myeloma. Sci Rep. 2016;6(36305). PMID: 27833099
  22. Kumar SA, Thakur C, Li L, Cui H, Chen F. Pathological and prognostic role of mdig in pancreatic cancer. Genes & cancer. 2017;8(7-8):650-8. PMID: 28966726

Poster presentations

Title of the paper: “Lineage tracing of metastasis in a Non-small cell lung cancer (NSCLC) mouse model”
Authors: Chitra Thakur, Fatih Ceteci, Ulf R Rapp
Presenter: Chitra Thakur
Conference: MRS-AACR (American Association for Cancer Research) Joint Conference on Metastasis and the Tumor Microenvironment, September 12th -15th, 2010 at the Sheraton Center City, Philadelphia, PA, USA.

Title of the paper: “Lineage tracing of metastasis in a non-small cell lung cancer (NSCLC) mouse model”
Authors: Chitra Thakur, Fatih Ceteci, Ulf R Rapp
Presenter: Chitra Thakur
Conference: Max Planck Institute’s Day on 18th May 2011 at the MPI Biochemistry, Martinsreid, Germany. 

Title of the paper: “Is Cancer initiation restricted to replicative cells?”
Authors: Chitra Thakur, Fatih Ceteci, Ulf R Rapp
Presenter: Chitra Thakur
Conference: 4th International Symposium organized by the students of Graduate School of Life Sciences, on “Research Revolution “University of Wurzburg, 26 -27 March 2009. 

Title of the paper: “mdig gene-environment interactions in human cancers”
Authors: Chitra Thakur, Fei Chen
Presenter: Chitra Thakur
Conference I: 2016 Graduate and Postdoctoral Research Symposium, at McGregor Memorial Conference Center, Wayne State University, Detroit, MI, USA, March 23, 2016
Conference II: 2016 Cancer Metabolism Symposium, University of Michigan- Biomedical Science Research Building (BSRB), May 25, 2016

Title of the paper: “Deficiency in mdig gene ameliorates silica-induced pulmonary fibrosis by altering balance between Th17 and Treg T cells and reducing lung infiltration of the Th17 cells”     
Authors: Chitra Thakur, Michael Wolfarth, Jiaying Sun, Yadong Zhang, Yongju Lu, Lori Battelli, Dale W. Porter, Fei Chen 
Presenter: Chitra Thakur
Conference: 11th Annual Research Forum, at Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI, USA, October 1, 2014.

Title of the paper: “Increased expression of mdig predicts poorer survival of the breast cancer patients”
Authors:  Chitra Thakur, Yongju Lu, Jiaying Sun, Miaomiao Yu, Bailing Chen, Fei Chen 
Presenter: Chitra Thakur
Conference: 11th Annual Research Forum, at Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI, USA, October 1, 2014. 

Title of the paper: “mdig gene-environment interactions in human cancers”
Authors:  Chitra Thakur, Fei Chen
Presenter: Chitra Thakur
Conference: AACR (American Association for Cancer Research) Precision Medicine Series: Opportunities and Challenges of Exploiting Synthetic Lethality in Cancer. January 4 - 7, 2017. Hard Rock Hotel San Diego, California
I earned 17.0 AMA PRA Category 1 Credits™ for this workshop 

Talks

“Lineage tracing of metastasis in a murine lung adenocarcinoma model” in the 11th Graduate Program of the Max Planck Institute of Biochemistry, Martinsreid Germany at Ringberg Castle, 21st -23rd March 2011.

“Realizing your full potential through a successful doctoral program” Graduate School of Life Sciences (GSLS), University of Würzburg, Germany:  Career Session, 19 July 2018. (via skype) 

“In Your Shoes: From International Student to Wayne State Employee” Purdy/Kresge Library # 150, 5265 Cass Detroit, MI, March 22nd 2019