Wayne State University
Olivia M Merkel Ph.D.

Olivia M Merkel Ph.D.

Assistant Professor, Pharmaceutical Sciences

Contact

313-577-1523
olivia.merkel@wayne.edu

Office location

EACPHS, Room 3132

Appointments

Assistant Professor, Department of Pharmaceutical Sciences

Degrees and Certifications

09/2009 08/2011 Postdoctoral Associate and Lecturer, Habilitation Candidate, Department of Pharmaceutics and Biopharmacy, Philipps-Universitt Marburg, Germany

02/2006 - 08/2009 PhD Student and Research Assistant, Department of Pharmaceutics and Biopharmacy, Philipps-Universitt Marburg, Germany, PI: Prof. Thomas Kissel, Thesis Title: Non-Viral Delivery of Nucleic Acids and Image-Guided Assessment of in vivo Performance of Multifunctional Nanomedicines

05/2005 - 10/2005 Pharmacy Practice Resident, Sonnen-Apotheke, Marburg, Germany Recognized as second half of residency.

1/2004 - 04/2005 Master Student and Research Assistant, Department of Pharmaceutics and Biopharmacy, Philipps-Universitt Marburg, Germany in collaboration with Martin-Luther-Universitt Halle-Wittenberg, Germany, PIs: Prof. Thomas Kissel and Prof. Karsten Mder, Thesis Title: Complexation of siRNA for Specific Knock Down of Luciferase in SKOV-3/Luc- and NIH-3T3-Cells Recognized as first half of residency.
10/2000 09/2004 Studies of Pharmacy, Philipps-Universitt Marburg, Germany

PhD in Pharmaceutics, Summa Cum Laude, 08/25/2009

MS in Pharmaceutics, With Distinction, 07/11/2006

Board Certificate as Pharmacist, 12/12/2005

Positions and Employment

09/2009 08/2011 Postdoctoral Associate and Lecturer, Habilitation Candidate, Department of Pharmaceutics and Biopharmacy, Philipps-Universitt Marburg, Germany
02/2006 - 08/2009 PhD Student and Research Assistant, Department of Pharmaceutics and Biopharmacy, Philipps-Universitt Marburg, Germany, PI: Prof. Thomas Kissel
02/2006 - 12/2010 Odd Locum Pharmacist, St. Jost Apotheke Marburg, Germany

05/2005 - 10/2005 Pharmacy Practice Resident, Sonnen-Apotheke, Marburg, Germany Recognized as second half of residency.
11/2004 - 04/2005 Master Student and Research Assistant, Department of Pharmaceutics and Biopharmacy, Philipps-Universitt Marburg, Germany Recognized as first half of residency.
02/2002 10/2003 Student Trainee in the Department of Quality Assurance, Fresenius Kabi Deutschland GmbH, Friedberg, Germany,
Practical training and establishment of an analytical HPLC-method
08/2001 Intern in the Department of Quality Assurance, Fresenius Kabi Deutschland GmbH, Friedberg, Germany, 
Practical training in spectroscopic methods (AAS, ICP)
03/2001 Intern in Pharmacy Practice, Rosen-Apotheke, Marburg, Germany
 

Awards and Honors

04/2015 Galenus Support awarded by the Galenus Foundation Vienna, Austria
11/2014 ERC Starting Grant
05/2014 Travel Grant German Scholars Organization e.V.
04/2014 Galenus Support awarded by the Galenus Foundation Vienna, Austria
03/2014 Dr. Jack Ryan Award endowed by the Dr. Morris S. Brent Fund
02/2014 Selected Host for the first North American Galenus Guest Professorship
04/2013 Galenus Support awarded by the Galenus Foundation Vienna, Austria
12/2012 Young Investigator Award, Eugene Applebaum College of Pharmacy and Health Sciences, WSU, Detroit, MI
10/2012 PHOENIX award for the best publication in Pharmaceutical Technology, Berlin, Germany
07/2012 Invited Participant in the Lindau Nobel Laureates Meeting, Lindau, Germany
04/2012 Travel Grant awarded by the Galenus Foundation Vienna, Austria
06/2011 Young Pharmaceutical Investigator Award, European Federation for Pharmaceutical Sciences (EUFEPS), PharmSciFair, Prague, Czech Republic
10/2010 New Talents for the Future of Nanomedicine, Nano DDS’10, Omaha, NE, USA
10/2010 Carl-Wilhelm-Scheele-Award for the best thesis in Pharmaceutical Sciences, German Pharmaceutical Society (DPhG), Braunschweig, Germany
08/2010 Travel Grant awarded by the German Academic Exchange Service (DAAD)
04/2010 Travel Grant awarded by the Galenus Foundation Vienna, Austria
12/2009 Research award for excellent scientific accomplishments, best PhD thesis at Philipps-Universität Marburg (2008-2009), awarded by the Board of Industry and Trade (IHK), Kassel, Germany, December 2009
10/2009 Travel Grant awarded by the German Society for Nuclear Medicine (Deutsche Gesellschaft für Nuklearmedizin e.V), Hannover, Germany, October 2009
05/2009 Promotion to Young Researchers awarded by the German Lung Foundation (Deutsche Lungenstiftung, e.V.), Hannover, Germany, May 2009
05/2009 GSK Travel Grant, GlaxoSmithKline Foundation, Munich, Germany
05/2009 ISAM student research award, Monterey, CA, USA
09/2008 Sponsored Participant, Globalization of Pharmaceutics Education Network, Leuven, Belgium
08/2008 Poster Prize Award, Chosen Flash Oral Presentation, Gordon Research Conference on Drug Carriers in Medicine & Biology, Big Sky, MT, USA
05/2008 Travel Grant Award, 7th International Symposium on Polymer Therapeutics, Valencia
09/2007 Best Nanomedicine Poster, 5th International Dendrimer Symposium, Toulouse


Honors to Lab Members:
05/2014 Barber Foundation Fellowships for Daniel Feldmann and Joshua Kovoor
05/2015 Predoctoral Fellowship in Cancer Research for Steven Jones awarded from the DeRoy Testamentary Foundation
04/2015 GRA in Nanomedicine for Yuran Xie awarded by the Office of the Vice President for Research
04/2015 Bradly-Alavi Fellowship for Steven Jones awarded by the Society of Nuclear Medicine
04/2015 Award for Oral Presentation at the Master Student Showcase for Ahmed El Mahmoudi
03/2015 Travel Grant for Steven Jones, European Molecular Imaging Meeting, Tübingen, Germany
01/2015 First place for basic science poster presentation at the MD Student Research Symposium for Vincent Lizzio
09/2014 Award for Best Talk at the Graduate Student Research Day for Steven Jones
07/2014 Highlight Presentation of Yuran Xie’s Poster from the Annual Meeting of the Controlled Release Society in the CAPSULE Newsletter
05/2014 Barber Foundation Fellowships for Nicholas Kolderman and Jameson Tockstein
05/2014 NIH T32 Fellowship for Steven Jones
05/2014 NIH BEST Fellowship for Daniel Feldmann
04/2014 Travel Grant for Steven Jones, 10thInternational Symposium on Polymer Therapeutics, Valencia, Spain
04/2014 Medical Student Summer Research Fellowship for Vincent Lizzio
01/2014 Angelika Burger Fellowship for Sara Movassaghian
12/2013 Nomination of Steven Jones for invited abstract submission to the 2014 Graduate Exhibition
10/2013 Innovation Fellowship for Dr. Venkatareddy Nadithe, awarded by the Wayne State University’s Technology Commercialization Office (TC)
08/2013 PROSA fellowship for Claudia Hildebrandt, awarded by the Ludwig-Maximilians-Universität München, Germany, for an internship abroad
05/2012 Junior Investigator Research Award for Dr. Na Hyung Kim, awarded for the best poster at the KCI Annual Research Retreat

Professional Memberships

American Association of Pharmaceutical Scientists (AAPS) since 2011
International Association for Pharmaceutical Technology (APV) since 2011
International Society of Aerosols in Medicine (ISAM) since 2009
European Society for Molecular Imaging (ESMI) since 2009
Controlled Release Society (CRS) since 2006
German Pharmaceutical Society (DPhG) since 2004
 

Areas of Expertise

Synthesis of bioconjugates, cell specific non-viral siRNA delivery, in vivo siRNA delivery, SPECT imaging, GFP-mice, pulmonary administration, nanotoxicity

Primary Research Interest

Development of targeted siRNA delivery systems for the treatment of asthma.

Recent Publications

1. Yuran Xie, Olivia M. Merkel*: Pulmonary Delivery of siRNA via Polymeric Vectors as Therapies of Asthma, Archiv der Pharmazie 2015, doi: 10.1002/ardp.20150012 (SCI: 1.40), Mini-Review *Corresponding Author
2. Rima Kandil, Olivia M. Merkel*: Therapeutic delivery of RNA: Diseases affecting the respiratory system, Pharmakon, 2015, 3 (4), 276-283 (SCI: 1.00), Review *Corresponding Author
3. Daniel P. Feldmann, Olivia M. Merkel*: The Advantages of Pulmonary Delivery of Therapeutic siRNA, Therapeutic Delivery 2015, 6 (4), 407-9, Editorial *Corresponding Author
4. Daniel G. Abebe, Rima Kandil, Teresa Kraus, Maha Elsayed, Olivia M. Merkel,* Tomoko Fujiwara*: Three-Layered Biodegradable Micelles Prepared by Two-Step Self-Assembly of PLA-PEI-PLA and PLA-PEG-PLA Triblock Copolymers for Efficient Gene Delivery System
Macromolecular Biosciences 2015, 15 (5), 698-711 (SCI: 3.65) *Corresponding Author
5. Sara Movassaghian, Olivia M. Merkel, Vladimir Torchilin: Applications of Polymer Micelles for Imaging and Drug Delivery, WIREs Nanomedicine & Nanobiotechnology 2015, doi: 10.1002/wnan.1332 (SCI: 5.68), Review
6. Venkatareddy Nadithe, Runhui Liu, Bryan Killinger, Sara Movassaghian, Na Hyung Kim, Anna Moszczynska, Kristyn S Masters, Samuel H Gellman, Olivia M Merkel*: Screening of Nylon-3 Polymers, a New Class of Cationic Amphiphiles for siRNA Delivery
Molecular Pharmaceutics 2015, 12 (2), 362-74 (SCI: 4.79) *Corresponding Author
7. R. Michael Dutescu, Claudia Panfil, Olivia M. Merkel, Norbert Schrage: Semifluorinated alkanes as a liquid drug carrier system for topical ocular drug delivery
European Journal of Pharmaceutics and Biopharmaceutics 2014, 88(1), 123–128 (SCI: 4.25)
8. Olivia M. Merkel*, Thomas Kissel: Quo vadis polyplex?, Journal of Controlled Release 2014, 190:415-23 (SCI: 7.26), Review*Corresponding Author
9. Olivia M. Merkel*, Israel Rubinstein, Thomas Kissel: siRNA Delivery to the lung: What's new?, Advanced Drug Delivery Reviews 2014, 75:112-28, (SCI: 12.89), Review *Corresponding Author
10. Maha Elsayed, Olivia M. Merkel*: 3D Nanoimprinting of cell culture scaffolds, Nanomedicine 2014, 9 (2), 349-366 (SCI: 5.82), Review*Corresponding Author
11. Maha Elsayed, Vincent Corrand, Vidula Kolhatkar, Yuran Xie, Na Hyung Kim, Rohit Kolhatkar, Olivia M Merkel*: The influence of oligospermine architecture on their suitability for siRNA delivery
Biomacromolecules 2014, 15 (4), 1299-310 (SCI: 5.79)*Corresponding Author
12. Thomas Endres, Mengyao Zheng, Ayse Kilic, Agnieszka Turowska, Moritz Beck-Broichsitter, HaraldRenz, Olivia M. Merkel*, Thomas Kissel: Amphiphilic biodegradable PEG-PCL-PEI triblock-copolymers for dual delivery of siRNA and quantum dots: in vitro and in vivo investigation of a FRET capable carrier system
Molecular Pharmaceutics 2014, 11 (4), 1273–1281 (SCI: 4.79)*Corresponding Author
13. Agnieszka Turowska, Damiano Librizzi, Nadja Baumgartl, Jens Kuhlmann, Tanja Dicke, Olivia Merkel, Helmut Höffken, Harald Renz, Holger Garn: Biodistribution of the GATA-3-specific DNAzyme hgd40 after inhalative exposure in mice, rats and dogs
Toxicology and Applied Pharmacology 2013, 272 (2), 365-72 (SCI: 3.63)
14. Na Hyung Kim, Venkatareddy Nadithe, Maha Elsayed, Olivia M Merkel*: Tracking and treating activated T cells
Journal of Drug Delivery Science and Technology 2013, 23 (1), 17-21 (SCI: 1.09) *Corresponding Author
15. Mengyao Zheng, Manuel Neeb, Giovanni Pavan, Andreas Schaper, Gerhard Klebe, Olivia M. Merkel*, Thomas Kissel*: Targeting the blind spot of polycationic nanocarrier-based siRNA delivery
ACS Nano 2012, 6 (11), 9447–9454 (SCI: 12.03) *Corresponding Authors
16. Mengyao Zheng, Damiano Librizzi, Ayse Kilic, Yu Liu, HaraldRenz, Olivia M. Merkel*, Thomas Kissel: Enhancing in vivo circulation and siRNA delivery with biodegradable hy-PEI-g-PCL-b-PEG copolymers
Biomaterials 2012, 33 (27), 6551-8 (SCI: 8.31) *Corresponding Author
17. Thomas Renette, Damiano Librizzi, Thomas Endres, Moritz Beck-Broichsitter, Nadja Bege, Olivia M. Merkel, Holger Petersen, Catherine Curdy, Thomas Kissel: Poly(ethylene carbonate) nanoparticles as carrier system for chemotherapy showing prolonged in vivo circulation and anti-tumor efficacy
Macromolecular Bioscience 2012, 12 (7), 970-8 (SCI: 3.65)
18. Nick D. Weber*, Olivia M. Merkel*, Thomas Kissel, María Angeles Muñoz-Fernández: PEGylated poly(ethylene imine) copolymer-delivered siRNA inhibits HIV replication in vitro,
Journal of Controlled Release 2012, 157 (1), 55-63 (SCI: 7.26)
19. Moritz Beck-Broichsitter, Olivia M. Merkel, Thomas Kissel: Controlled pulmonary drug and gene delivery using polymeric nano-carriers, Journal of Controlled Release 2012, 161, 214–224 (SCI: 7.26), Review
20. Olivia M. Merkel, Mengyao Zheng, Heiko Debus, Thomas Kissel: Non-viral Vectors in Pulmonary Gene Delivery, Bioconjugate Chemistry 2012, 23 (1), 3-20, (SCI: 4.58), Review, Chosen for Cover
21. Olivia M. Merkel, Thomas Kissel: Nonviral Pulmonary delivery of siRNA,
Accounts of Chemical Research 2012, 45 (7), 961-70 (SCI: 24.35)
22. Mengyao Zheng, Yu Liu, Olga Samsonova, Thomas Endres, Olivia M. Merkel, Thomas Kissel: The influence of graft density of biodegradable hy-PEI-g-PCL-b-PEG copolymers on their performance as non-viral gene delivery vectors, International Journal of Pharmaceutics 2012, 427(1):80-7 (SCI: 3.79)
23. Yu Liu, Olga Samsonova, Brian Sproat, Olivia M. Merkel, Thomas Kissel: Biophysical characterization of hyper-branched polyethylenimine-graft- polycaprolactone-block-mono-methoxyl-poly(ethylene glycol) copolymers (hy-PEI-PCL-mPEG) for siRNA delivery, Journal of Controlled Release 2011, 153 (3), 262-8 (SCI: 7.26)
24. Olga Samsonova, Christian Pfeiffer, Markus Hellmund, Olivia M. Merkel*, Thomas Kissel: pDMAEMA-pHEMA polymers as potential non-viral carriers for gene transfer synthesized via RAFT-Polymerisation, Polymers 2011, 3, 693-718, (SCI: 2.51) *Corresponding Author
25. Olivia M. Merkel, Rudolf Urbanics, Peter Bedocs, Zoltán Rozsnyay, László Rosivall, Miklós Toth, Thomas Kissel, Janos Szebeni: In vitro and in vivo Complement Activation and Related Anaphylactic Effects Associated with Polyethylenimine and Polyethylenimine-graft-poly(ethylene glycol) Block Copolymers, Biomaterials 2011, 32 (21), 4936-42 (SCI: 8.31)
26. Nadja Bege, Thomas Renette, Mirko Jansch, Regina Reul, Olivia M. Merkel, Holger Petersen, Catherine Curdy, Rainer H. Müller, Thomas Kissel: Biophysical Poly(ethylene carbonate) nanoparticles: A novel biodegradable carrier system with “stealth potential, Macromolecular Bioscience 2011, 11 (7), 897-904 (SCI: 3.65)
27. Olivia M. Merkel*, Mengyao Zheng, Meredith Mintzer, Giovanni M. Pavan, Damiano Librizzi, Helmut Höffken, Andrea Danani, Eric Simanek, Thomas Kissel: Molecular modeling and in vivo imaging can identify successful flexible triazine dendrimer-based siRNA delivery systems, Journal of Controlled Release 2011, 153 (1), 23-33 (SCI: 7.26) *Corresponding Author, chosen for JCR Topic Collection on Molecular Imaging
28. Andrea Beyerle, Andrea Braun, Olivia M. Merkel, Felix Koch, Thomas Kissel, Tobias Stoeger: Comparative in vivo study of poly(ethylene imine)/sirna complexes for pulmonary delivery in mice, Journal of Controlled Release 2011, 151 (1), 51-56 (SCI: 7.26)
29. Olivia M. Merkel, Andrea Beyerle, Benedikt Beckmann, Mengyao Zheng, Roland Hartmann, Tobias Stoeger, Thomas Kissel: Polymer-related off-target effects in non-viral siRNA delivery, Biomaterials 2011, 32, 2388-2398 (SCI: 8.31)
30. Olivia M. Merkel*, Meredith A. Mintzer*, Damiano Librizzi, Olga Samsonova, Tanja Dicke, Brian Sproat, Peter J. Barth, Holger Garn, Eric E. Simanek, and Thomas Kissel: Triazine dendrimers as non-viral vectors for in vitro and in vivo RNAi: The effects of peripheral groups and core structure on biological activity, Molecular Pharmaceutics 2010, 7 (4), 969–983 (SCI: 4.79)
31. Giovanni M. Pavan, Meredith Mintzer, Eric Simanek, Olivia M. Merkel, Thomas Kissel, Andrea Danani: Computational Insights into the Interactions between DNA and siRNA with “Rigid and “Flexible Triazine Dendrimers, Biomacromolecules 2010, 11 (3), 721-730 (SCI: 5.79)
32. Andrea Beyerle, Olivia Merkel, Tobias Stoeger, Thomas Kissel: PEGylation affects cytotoxicity and biocompatibility of poly(ethylene imine) for lung application : structure-function relationships, Toxicology and Applied Pharmacology 2010, 242 (2), 146-154 (SCI: 3.63)
33. Olivia M. Merkel*, Meredith Mintzer*, Eric Simanek, Thomas Kissel: Triazine dendrimers as nonviral gene delivery systems: effects of molecular structure on biological activity, Bioconjugate Chemistry 2009, 20 (9), 1799-806 (SCI: 4.82)
34. Olivia M. Merkel*, Meredith A. Mintzer, Thomas Kissel, Eric Simanek: Perfectly Shaped siRNA Delivery, Therapeutic Delivery 2010, 1 (6), 737-742, Invited Editorial, *Corresponding Author
35. Olivia M. Merkel, Andrea Beyerle, Damiano Librizzi, Andreas Pfestroff, Thomas M. Behr, Brian Sproat, Peter J. Barth, Thomas Kissel:Nonviral siRNA delivery to the lung: investigation of PEG-PEI polyplexes and their in vivo performance, Molecular Pharmaceutics 2009, 6 (4), 1246-60 (SCI: 4.79)
36. Yu Liu, Juliane Nguyen, Terry Steele, Olivia Merkel, Thomas Kissel: A new synthesis method and degradation of hyper-branched polyethylenimine grafted polycaprolactone block mono-methoxyl poly (ethylene glycol) copolymers (hy-PEI-g-PCL-b-mPEG) as potential DNA delivery vectors, Polymer 2009, 50 (16), 3895-3904 (SCI: 3.77)
37. Meredith A. Mintzer*, Olivia M. Merkel*, Thomas Kissel, Eric Simanek: Polycationic triazine-based dendrimers: Effect of peripheral groups on transfection efficiency, New Journal of Chemistry 2009, 33 (9), 1918-25 (SCI: 3.16)
38. Olivia M. Merkel, Oliver Germershaus, Carol K. Wada, Peter J. Tarcha, Thomas Merdan and Thomas Kissel: Integrin alpha-v-beta-3 targeted gene delivery using RGD peptidomimetic conjugates with copolymers of PEGylated poly(ethylene imine), Bioconjugate Chemistry 2009, 20 (6), 1270-1280 (SCI: 4.82)
39. Olivia M. Merkel, Damiano Librizzi, Andreas Pfestroff, Tino Schurrat, Kevin Buyens, Niek N. Sanders, Stefaan DeSmedt, Martin Béhé and Thomas Kissel: Stability of siRNA polyplexes from poly(ethylenimine) and poly(ethylenimine)-g-poly(ethylene glycol) under in vivo conditions: Effects on pharmacokinetics and biodistribution measured by Fluorescence Fluctuation Spectroscopy and Single Photon Emission Computed Tomography (SPECT) imaging, Journal of Controlled Release 2009, 138 (2), 148-59 (SCI: 7.26), chosen for JCR Topic Collection on Molecular Imaging
40. Anastasia Malek, Olivia Merkel, Ludger Fink, Frank Czubayko, Thomas Kissel and Achim Aigner: In vivo pharmacokinetics, tissue distribution and underlying mechanisms of various PEI(-PEG)/siRNA complexes, Toxicology and applied pharmacology 2009, 236 (1), 97-108 (SCI: 3.66)
41. Olivia M. Merkel, Damiano Librizzi, Andreas Pfestroff, Tino Schurrat, Martin Béhé and Thomas Kissel: In vivo SPECT and real-time gamma camera imaging of biodistribution and pharmacokinetics of siRNA delivery after novel radio labeling and purification, Bioconjugate Chemistry 2009, 20 (1), 174-182 (SCI: 4.82)
42. Juliane Nguyen, Terry W.J. Steele, Olivia Merkel, Regina Reul and Thomas Kissel: Fast degrading polyesters as siRNA nano-carriers for pulmonary gene therapy, Journal of Controlled Release 2008, 132, 243–251 (SCI: 7.26)
43. Shirui Mao, Michael Neu, Oliver Germershaus, Olivia Merkel, Johannes Sitterberg, Udo Bakowsky, Thomas Kissel: Influence of polyethylene glycol chain length on the physicochemical and biological properties of poly (ethylene imine)-graft-poly (ethylene glycol) block copolymer/ siRNA polyplexes, Bioconjugate Chemistry 2006, 17 (5), 1209 -1218 (SCI: 4.82)

Book Chapters
Steven K Jones, Olivia M. Merkel: Indium-labeling of siRNA for small animal SPECT imaging, Invited Book Chapter in " RNA lmaging: Methods and Protocols, Methods Mol Biol. 2015 *Corresponding Author
Maha Elsayed, Olivia M. Merkel: Inhaled Delivery of siRNA, eBook Chapter in "Oligonucleotide Nanomedicines: Challenges and Opportunities, Future Science Press, Eds: Olivia Merkel and Mansoor Amiji *Corresponding Author
Mengyao Zheng, Thomas Kissel, Olivia M. Merkel: Self-assembly of siRNA containing nanoparticles, Book Chapter in "RNA Nanotechnology and Therapeutics, CRC Press, July 2013, ISBN 9781466505667 *Corresponding Author
Olivia M. Merkel*, Leigh Marsh, Holger Garn, Thomas Kissel: Flow cytometry-based cell type-specific assessment of target regulation by pulmonary siRNA delivery, Methods Mol Biol. 2013, 948, 263-73, *Corresponding Author
Meredith A. Mintzer, Olivia M. Merkel, Thomas Kissel, Eric Simanek: Triazine dendrimers in gene and siRNA delivery: progress, challenges, and opportunities, Invited Book Chapter in "Dendrimer-based Drug Delivery Systems: from Theory to Practice", John Wiley Sons., Inc., July 2012, ISBN-10: 0-470-46005-9
 

Research Connect link

https://researchconnect.wayne.edu/en/persons/olivia-merkel

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