Wayne State University

Aim Higher

Faculty Biography
 

Olivia M Merkel, PhD


(313) 577-1523
(313) 577-6511
olivia.merkel@wayne.edu
http://sites.google.com/site/merkellab/
EACPHS Room 3132

Appointments

Assistant Professor, Department of Pharmaceutical Sciences


Education Training

09/2009 – 08/2011         Postdoctoral Associate and Lecturer, Habilitation Candidate, Department of Pharmaceutics and Biopharmacy, Philipps-Universität Marburg, Germany

02/2006 - 08/2009          PhD Student and Research Assistant, Department of Pharmaceutics and Biopharmacy, Philipps-Universität Marburg, Germany, PI: Prof. Thomas Kissel, Thesis Title: „Non-Viral Delivery of Nucleic Acids and Image-Guided Assessment of in vivo Performance of Multifunctional Nanomedicines

05/2005 - 10/2005          Pharmacy Practice Resident, Sonnen-Apotheke, Marburg, Germany – Recognized as second half of residency.

11/2004 - 04/2005          Master Student and Research Assistant, Department of Pharmaceutics and Biopharmacy, Philipps-Universität Marburg, Germany in collaboration with Martin-Luther-Universität Halle-Wittenberg, Germany, PIs: Prof. Thomas Kissel and Prof. Karsten Mäder, Thesis Title: „Complexation of siRNA for Specific Knock Down of Luciferase in SKOV-3/Luc- and NIH-3T3-Cells– Recognized as first half of residency.

10/2000 – 09/2004           Studies of Pharmacy, Philipps-Universität Marburg, Germany


Degrees and Certifications

PhD in Pharmaceutics, Summa Cum Laude, 08/25/2009

MS in Pharmaceutics, With Distinction, 07/11/2006

Board Certificate as Pharmacist, 12/12/2005


Positions and Employment

09/2009 – 08/2011           Postdoctoral Associate and Lecturer, Habilitation Candidate, Department of Pharmaceutics and Biopharmacy, Philipps-Universität Marburg, Germany

02/2006 - 08/2009           PhD Student and Research Assistant, Department of Pharmaceutics and Biopharmacy, Philipps-Universität Marburg, Germany, PI: Prof. Thomas Kissel

02/2006 - 12/2010           Odd Locum Pharmacist, St. Jost Apotheke Marburg, Germany

05/2005 - 10/2005           Pharmacy Practice Resident, Sonnen-Apotheke, Marburg, Germany – Recognized as second half of residency.

11/2004 - 04/2005           Master Student and Research Assistant, Department of Pharmaceutics and Biopharmacy, Philipps-Universität Marburg, Germany – Recognized as first half of residency.

02/2002 – 10/2003           Student Trainee in the Department of Quality Assurance, Fresenius Kabi Deutschland GmbH, Friedberg, Germany,
Practical training and establishment of an analytical HPLC-method

08/2001                               Intern in the Department of Quality Assurance, Fresenius Kabi Deutschland GmbH, Friedberg, Germany,
Practical training in spectroscopic methods (AAS, ICP)

03/2001                               Intern in Pharmacy Practice, Rosen-Apotheke, Marburg, Germany


Other Experience Professional Memberships

·         American Association of Pharmaceutical Scientists (AAPS) since 2011

·         International Association for Pharmaceutical Technology (APV) since 2011

·         International Society of Aerosols in Medicine (ISAM) since 2009

·         European Society for Molecular Imaging (ESMI) since 2009

·         Controlled Release Society (CRS) since 2006

·         German Pharmaceutical Society (DPhG) since 2004


Awards and Honors

05/2014                               Travel Grant German Scholars Organization e.V.

04/2014                               Galenus Support awarded by the Galenus Foundation Vienna, Austria

03/2014                               Dr. Jack Ryan Award endowed by the Dr. Morris S. Brent Fund

02/2014                               Selected Host for the first North American Galenus Guestprofessorship

04/2013                               Galenus Support awarded by the Galenus Foundation Vienna, Austria

12/2012                               Young Investigator Award, Eugene Applebaum College of Pharmacy and Health Sciences, WSU, Detroit, MI

10/2012                               PHOENIX award for the best publication in Pharmaceutical Technology, Berlin, Germany

07/2012                               Invited Participant in the Lindau Nobel Laureates Meeting, Lindau, Germany

04/2012                               Travel Grant awarded by the Galenus Foundation Vienna, Austria

06/2011                               Young Pharmaceutical Investigator Award, European Federation for Pharmaceutical Sciences (EUFEPS), PharmSciFair, Prague, Czech Republic

10/2010                               New Talents for the Future of Nanomedicine, Nano DDS’10, Omaha, NE, USA

10/2010                               Carl-Wilhelm-Scheele-Award for the best thesis in Pharmaceutical Sciences, German Pharmaceutical Society (DPhG), Braunschweig, Germany

08/2010                               Travel Grant awarded by the German Academic Exchange Service (DAAD)

04/2010                               Travel Grant awarded by the Galenus Foundation Vienna, Austria

12/2009                               Research award for excellent scientific accomplishments, best PhD thesis at Philipps-Universität Marburg (2008-2009), awarded by the Board of Industry and Trade (IHK), Kassel, Germany, December 2009

10/2009                               Travel Grant awarded by the German Society for Nuclear Medicine (Deutsche Gesellschaft für Nuklearmedizin e.V), Hannover, Germany, October 2009

05/2009                               Promotion to Young Researchers awarded by the German Lung Foundation (Deutsche Lungenstiftung, e.V.), Hannover, Germany, May 2009

05/2009                                GSK Travel Grant, GlaxoSmithKline Foundation, Munich, Germany

05/2009                               ISAM student research award, Monterey, CA, USA

09/2008                               Sponsored Participant, Globalization of Pharmaceutics Education Network, Leuven, Belgium

08/2008                               Poster Prize Award, Chosen Flash Oral Presentation, Gordon Research Conference on Drug Carriers in Medicine & Biology, Big Sky, MT, USA

05/2008                               Travel Grant Award, 7th International Symposium on Polymer Therapeutics, Valencia

09/2007                                Best Nanomedicine Poster, 5th International Dendrimer Symposium, Toulouse

Honors to Lab Members:

05/2012                               Junior Investigator Research Award for Dr. Na Hyung Kim, awarded for the best poster at the KCI Annual Research Retreat

10/2013                               Innovation Fellowship for Dr. Venkatareddy Nadithe, awarded by the Wayne State University’s Technology Commercialization Office (TC) 

08/2013                               PROSA fellowship for Claudia Hildebrandt, awarded by the Ludwig-Maximilians-Universität München, Germany, for an internship abroad

12/2013                               Nomination of Steven Jones for invited abstract submission to the 2014 Graduate Exhibition

01/2014                               Angelika Burger Fellowship for Sara Movassaghian

04/2014                               Medical Student Summer Research Fellowship for Vincent Lizzio

04/2014                               Travel Grant for Steven Jones, 10th International Symposium on Polymer Therapeutics, Valencia

05/2014                               BEST Fellowship for Daniel Feldmann

05/2014                               NIH T32 Fellowship for Steven Jones

05/2014                               Barber Foundation Fellowships for Nicholas Kolderman and Jameson Tockstein

 


Recent Publications

1.       Olivia M. Merkel*, Thomas Kissel: Quo vadis polyplex?, Journal of Controlled Release 2014, doi:10.1016/j.jconrel.2014.06.009 (SCI: 7.63), Review

2.       Olivia M. Merkel*, Israel Rubinstein, Thomas Kissel: siRNA Delivery to the lung: What's new?, Advanced Drug Delivery Reviews 2014, doi: 10.1016/j.addr.2014.05.018, (SCI: 12.89), Review

3.       R. Michael Dutescu, Claudia Panfil, Olivia M. Merkel, Norbert Schrage: Semifluorinated alkanes as a liquid drug carrier system for topical ocular drug delivery

European Journal of Pharmaceutics and Biopharmaceutics 2014, doi:10.1016/j.ejpb.2014.05.009 (SCI: 3.826)

4.       Maha Elsayed, Olivia M. Merkel*: 3D Nanoimprinting of cell culture scaffolds, Nanomedicine 2014, 9 (2), 349-366 (SCI: 5.26), Review

5.       Maha Elsayed, Vincent Corrand, Vidula Kolhatkar, Yuran Xie, Na Hyung Kim, Rohit Kolhatkar, Olivia M Merkel*: The influence of oligospermine architecture on their suitability for siRNA delivery  

Biomacromolecules 2014, 15 (4), 1299-310 (SCI: 5.37)*Corresponding Author

6.       Thomas Endres, Mengyao Zheng, Ayse Kilic, Agnieszka Turowska, Moritz Beck-Broichsitter, Harald Renz, Olivia M. Merkel*, Thomas Kissel: Amphiphilic biodegradable PEG-PCL-PEI triblock-copolymers for dual delivery of siRNA and quantum dots: in vitro and in vivo investigation of a FRET capable carrier system

Molecular Pharmaceutics 2014, 11 (4), 1273–1281 (SCI: 4.57)*Corresponding Author

7.       Agnieszka Turowska, Damiano Librizzi, Nadja Baumgartl, Jens Kuhlmann, Tanja Dicke, Olivia Merkel, Helmut Höffken, Harald Renz, Holger Garn: Biodistribution of the GATA-3-specific DNAzyme hgd40 after inhalative exposure in mice, rats and dogs

Toxicology and Applied Pharmacology 2013, 272 (2), 365-72 (SCI: 4.00)

8.       Na Hyung Kim, Venkatareddy Nadithe, Maha Elsayed, Olivia M Merkel*: Tracking and treating activated T cells

Journal of Drug Delivery Science and Technology 2013, 23 (1), 17-21 (SCI: 1.09) *Corresponding Author

9.       Mengyao Zheng, Manuel Neeb, Giovanni Pavan, Andreas Schaper, Gerhard Klebe, Olivia M. Merkel*, Thomas Kissel*: Targeting the blind spot of polycationic nanocarrier-based siRNA delivery

ACS Nano 2012, 6 (11), 9447–9454 (SCI: 12.06) *Corresponding Authors

10.    Mengyao Zheng, Damiano Librizzi, Ayse Kilic, Yu Liu, Harald Renz, Olivia M. Merkel*, Thomas Kissel: Enhancing in vivo circulation and siRNA delivery with biodegradable hy-PEI-g-PCL-b-PEG copolymers

Biomaterials 2012, 33 (27), 6551-8 (SCI: 7.60) *Corresponding Author

11.    Moritz Beck-Broichsitter, Olivia M. Merkel, Thomas Kissel: Controlled pulmonary drug and gene delivery using polymeric nano-carriers, Journal of Controlled Release 2012, 161, 214–224 (SCI: 7.36), Review

12.    Olivia M. Merkel, Mengyao Zheng, Heiko Debus, Thomas Kissel: Non-viral Vectors in Pulmonary Gene Delivery, Bioconjugate Chemistry 2012, 23 (1), 3-20, (SCI: 4.58), Review, Chosen for Cover

13.    Thomas Renette, Damiano Librizzi, Thomas Endres, Moritz Beck-Broichsitter, Nadja Bege, Olivia M. Merkel, Holger Petersen, Catherine Curdy, Thomas Kissel: Poly(ethylene carbonate) nanoparticles as carrier system for chemotherapy showing prolonged in vivo circulation and anti-tumor efficacy

Macromolecular Bioscience 2012, 12 (7), 970-8 (SCI: 3.74)

14.    Nick D. Weber*, Olivia M. Merkel*, Thomas Kissel, María Angeles Muñoz-Fernández: PEGylated poly(ethylene imine) copolymer-delivered siRNA inhibits HIV replication in vitro,

Journal of Controlled Release 2012, 157 (1), 55-63 (SCI: 7.63)

15.    Olivia M. Merkel, Thomas Kissel: Nonviral Pulmonary delivery of siRNA,

Accounts of Chemical Research 2012, 45 (7), 961-70 (SCI: 20.83)

16.    Mengyao Zheng, Yu Liu, Olga Samsonova, Thomas Endres, Olivia M. Merkel, Thomas Kissel: The influence of graft density of biodegradable hy-PEI-g-PCL-b-PEG copolymers on their performance as non-viral gene delivery vectors, International Journal of Pharmaceutics 2012, 427(1):80-7 (SCI: 3.46)

17.    Yu Liu, Olga Samsonova, Brian Sproat, Olivia M. Merkel, Thomas Kissel: Biophysical characterization of hyper-branched polyethylenimine-graft- polycaprolactone-block-mono-methoxyl-poly(ethylene glycol) copolymers (hy-PEI-PCL-mPEG) for siRNA delivery, Journal of Controlled Release 2011, 153 (3), 262-8 (SCI: 7.63)

18.    Olga Samsonova, Christian Pfeiffer, Markus Hellmund, Olivia M. Merkel*, Thomas Kissel: pDMAEMA-pHEMA polymers as potential non-viral carriers for gene transfer synthesized via RAFT-Polymerisation, Polymers 2011, 3, 693-718, (SCI: 1.69)  *Corresponding Author

19.    Olivia M. Merkel, Rudolf Urbanics, Peter Bedocs, Zoltán Rozsnyay, László Rosivall, Miklós Toth, Thomas Kissel, Janos Szebeni: In vitro and in vivo Complement Activation and Related Anaphylactic Effects Associated with Polyethylenimine and Polyethylenimine-graft-poly(ethylene glycol) Block Copolymers, Biomaterials 2011, 32 (21), 4936-42 (SCI: 7.60)

20.    Nadja Bege, Thomas Renette, Mirko Jansch, Regina Reul, Olivia M. Merkel, Holger Petersen, Catherine Curdy, Rainer H. Müller, Thomas Kissel: Biophysical Poly(ethylene carbonate) nanoparticles: A novel biodegradable carrier system with “stealth” potential, Macromolecular Bioscience 2011, 11 (7), 897-904 (SCI: 3.74)

21.    Olivia M. Merkel*, Mengyao Zheng, Meredith Mintzer, Giovanni M. Pavan, Damiano Librizzi, Helmut Höffken, Andrea Danani, Eric Simanek, Thomas Kissel: Molecular modeling and in vivo imaging can identify successful flexible triazine dendrimer-based siRNA delivery systems, Journal of Controlled Release 2011, 153 (1), 23-33 (SCI: 7.63) *Corresponding Author, chosen for JCR Topic Collection on Molecular Imaging

22.    Andrea Beyerle, Andrea Braun, Olivia M. Merkel, Felix Koch, Thomas Kissel, Tobias Stoeger: Comparative in vivo study of poly(ethylene imine)/sirna complexes for pulmonary delivery in mice, Journal of Controlled Release 2011, 151 (1), 51-56 (SCI: 7.63)

23.    Olivia M. Merkel, Andrea Beyerle, Benedikt Beckmann, Mengyao Zheng, Roland Hartmann, Tobias Stoeger, Thomas Kissel: Polymer-related off-target effects in non-viral siRNA delivery, Biomaterials 2011, 32, 2388-2398 (SCI: 7.60)

24.    Olivia M. Merkel*, Meredith A. Mintzer*, Damiano Librizzi, Olga Samsonova, Tanja Dicke, Brian Sproat, Peter J. Barth, Holger Garn, Eric E. Simanek, and Thomas Kissel: Triazine dendrimers as non-viral vectors for in vitro and in vivo RNAi: The effects of peripheral groups and core structure on biological activity, Molecular Pharmaceutics 2010, 7 (4), 969–983 (SCI: 4.57)

25.    Giovanni M. Pavan, Meredith Mintzer, Eric Simanek, Olivia M. Merkel, Thomas Kissel, Andrea Danani: Computational Insights into the Interactions between DNA and siRNA with “Rigid” and “Flexible” Triazine Dendrimers, Biomacromolecules 2010, 11 (3), 721-730  (SCI: 5.37)

26.    Andrea Beyerle, Olivia Merkel, Tobias Stoeger, Thomas Kissel: PEGylation affects cytotoxicity and biocompatibility of poly(ethylene imine) for lung application : structure-function relationships, Toxicology and Applied Pharmacology 2010, 242 (2), 146-154 (SCI: 4.00)

27.    Olivia M. Merkel*, Meredith A. Mintzer, Thomas Kissel, Eric Simanek: Perfectly Shaped siRNA Delivery, Therapeutic Delivery 2010, 1 (6), 737-742, Invited Editorial, *Corresponding Author

28.    Olivia M. Merkel*, Meredith Mintzer*, Eric Simanek, Thomas Kissel: Triazine dendrimers as nonviral gene delivery systems: effects of molecular structure on biological activity, Bioconjugate Chemistry 2009, 20 (9), 1799-806 (SCI: 4.58)

29.    Olivia M. Merkel, Andrea Beyerle, Damiano Librizzi, Andreas Pfestroff, Thomas M. Behr, Brian Sproat, Peter J. Barth, Thomas Kissel: Nonviral siRNA delivery to the lung: investigation of PEG-PEI polyplexes and their in vivo performance, Molecular Pharmaceutics 2009, 6 (4), 1246-60 (SCI: 4.57)

30.    Yu Liu, Juliane Nguyen, Terry Steele, Olivia Merkel, Thomas Kissel: A new synthesis method and degradation of hyper-branched polyethylenimine grafted polycaprolactone block mono-methoxyl poly (ethylene glycol) copolymers (hy-PEI-g-PCL-b-mPEG) as potential DNA delivery vectors, Polymer 2009, 50 (16), 3895-3904 (SCI : 3.38)

31.    Meredith A. Mintzer*, Olivia M. Merkel*, Thomas Kissel, Eric Simanek: Polycationic triazine-based dendrimers:  Effect of peripheral groups on transfection efficiency, New Journal of Chemistry 2009, 33 (9), 1918-25 (SCI: 2.97)

32.    Olivia M. Merkel, Oliver Germershaus, Carol K. Wada, Peter J. Tarcha, Thomas Merdan and Thomas Kissel: Integrin alpha-v-beta-3 targeted gene delivery using RGD peptidomimetic conjugates with copolymers of PEGylated poly(ethylene imine), Bioconjugate Chemistry 2009, 20 (6), 1270-1280 (SCI: 4.58)

33.    Olivia M. Merkel, Damiano Librizzi, Andreas Pfestroff, Tino Schurrat, Kevin Buyens, Niek N. Sanders, Stefaan DeSmedt, Martin Béhé and Thomas Kissel: Stability of siRNA polyplexes from poly(ethylenimine) and poly(ethylenimine)-g-poly(ethylene glycol) under in vivo conditions: Effects on pharmacokinetics and biodistribution measured by Fluorescence Fluctuation Spectroscopy and Single Photon Emission Computed Tomography (SPECT) imaging, Journal of Controlled Release 2009, 138 (2), 148-59 (SCI: 7.63), chosen for JCR Topic Collection on Molecular Imaging

34.    Anastasia Malek, Olivia Merkel, Ludger Fink, Frank Czubayko, Thomas Kissel and Achim Aigner: In vivo pharmacokinetics, tissue distribution and underlying mechanisms of various PEI(-PEG)/siRNA complexes, Toxicology and applied pharmacology 2009, 236 (1), 97-108 (SCI: 4.00)

35.    Olivia M. Merkel, Damiano Librizzi, Andreas Pfestroff, Tino Schurrat, Martin Béhé and Thomas Kissel: In vivo SPECT and real-time gamma camera imaging of biodistribution and pharmacokinetics of siRNA delivery after novel radio labeling and purification, Bioconjugate Chemistry 2009, 20 (1), 174-182 (SCI: 4.58)

36.    Juliane Nguyen, Terry W.J. Steele, Olivia Merkel, Regina Reul and Thomas Kissel: Fast degrading polyesters as siRNA nano-carriers for pulmonary gene therapy, Journal of Controlled Release 2008, 132, 243–251 (SCI: 7.63)

37.    Shirui Mao, Michael Neu, Oliver Germershaus, Olivia Merkel, Johannes Sitterberg, Udo Bakowsky, Thomas Kissel: Influence of polyethylene glycol chain length on the physicochemical and biological properties of poly (ethylene imine)-graft-poly (ethylene glycol) block copolymer/ siRNA polyplexes, Bioconjugate Chemistry 2006, 17 (5), 1209 -1218 (SCI: 4.58)

 


Primary Research Title

Non-viral siRNA delivery for anti-inflammatory therapy


Primary Research Interest

Development of targeted siRNA delivery systems for the treatment of asthma.


Secondary Research Title

Cancer Immunotherapy & Theragnostics


Secondary Research Interest

My recent goal is to develop novel safe and target-specific theragnostic nanomedicines. Specifically, we plan to synthesize and genetically engineer novel nanoscale delivery systems for siRNA which on the one hand have a strong affinity to the cells to be treated. On the other hand, and due to their high affinity to the target cells, these delivery systems shall be investigated for their ability to serve as diagnostic tool after radiolabeling by detecting circulating or distant target cells, e.g. circulating tumor cells or metastases.


Areas of Expertise

Synthesis of bioconjugates, cell specific non-viral siRNA delivery, in vivo siRNA delivery, SPECT imaging, GFP-mice, pulmonary administration, nanotoxicity